QIAGEN Plasmid Purification Handbook

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Third Edition November 2005 QIAGEN® Plasmid Purification Handbook QIAGEN Plasmid Mini, Midi, Maxi, Mega, and Giga Kits For purification of ultrapure, transfection grade plasmid DNA W W W. Q I A G E N . C O M Trademarks: QIAGEN®, Effectene®, EndoFree®, HiSpeed®, PolyFect®, SuperFect® (QIAGEN Group); DH5a® (Invitrogen Corp.); pBluescript® (Stratagene Inc.); Triton® (Rohm and Haas Company); pGEM® (Promega Corp.); Sorvall® (Sorvall Products, L. P.). Registered names, trademarks, etc. used in th
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  QIAGEN ® Plasmid PurificationHandbook  QIAGEN Plasmid Mini, Midi, Maxi, Mega,and Giga KitsFor purification of ultrapure, transfectiongrade plasmid DNA Third EditionNovember 2005  WWW.QIAGEN.COM  Trademarks: QIAGEN ® , Effectene ® , EndoFree ® , HiSpeed ® , PolyFect ® , SuperFect ® (QIAGEN Group); DH5 a ® (Invitrogen Corp.);pBluescript ® (Stratagene Inc.); Triton ® (Rohm and Haas Company); pGEM ® (Promega Corp.); Sorvall ® (Sorvall Products, L.P.).Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to be consideredunprotected by law.© 2005 QIAGEN, all rights reserved.  Contents Kit Contents4Storage5Quality Control5Product Use Limitations6Product Warranty and Satisfaction Guarantee6Technical Assistance6Safety Information7Introduction8 Principle and procedure8 Equipment and Reagents to Be Supplied by User10Important Notes11ProtocolsPlasmid or Cosmid DNA Purification UsingQIAGEN Plasmid Mini Kit15Plasmid or Cosmid DNA Purification UsingQIAGEN Plasmid Midi and Maxi Kits19Plasmid or Cosmid DNA Purification UsingQIAGEN Plasmid Mega and Giga Kits24 Very Low-Copy Plasmid/Cosmid Purification UsingQIAGEN-tip 100 or QIAGEN-tip 50029Troubleshooting Guide35 Appendix A: Agarose Gel Analysis of the Purification Procedure41 Appendix B: Composition of Buffers44Ordering Information46QIAGEN Distributors51  Kit Contents QIAGENMiniMiniMidiMidiMaxiMaxiMaxiPlasmid Kit(25)(100)(25)(100)(10)(25)(100)Catalog no. 12123121251214312145121621216312165 QIAGEN-tip25100–––––20QIAGEN-tip––25100–––100QIAGEN-tip––––10254 x50025Buffer P120 ml40 ml110 ml440 ml110 ml280 ml4 x280 mlBuffer P220 ml40 ml110 ml440 ml110 ml280 ml4 x280 mlBuffer P320 ml40 ml110 ml440 ml110 ml280 ml4 x280 mlBuffer QBT40 ml110 ml110 ml440 ml110 ml280 ml4 x280 mlBuffer QC120 ml480 ml3 x 3 x 3 x4 x 16 x190 ml750 m240 ml425 ml425 mlBuffer QF30 ml110 ml170 ml2 x 170 ml420 ml4 x280 ml420 mlRNase A*2 mg4 mg11 mg44 mg11 mg28 mg4 x28 mgLyseBlue20 µl40 µl110 µl440 µl110 µl280 µl4 x280 µlHandbook1111111 *Provided in a 10 mg/ml or 100 mg/ml solution.
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